Ex vivo and in vivo fluorescence detection and imaging of adenosine triphosphate

Abstract Background Ex vivo and in detection imaging of adenosine triphosphate (ATP) is critically important for the diagnosis treatment diseases, which still remains challenges up to present. Results We herein demonstrate that ATP could be fluorescently detected imaged ex vivo. In particular, we fabricate a kind fluorescent probes, are made titanium carbide (TC) nanosheets modified with ROX-tagged ATP-aptamer (TC/Apt). constructed TC/Apt, TC shows superior quenching efficiency against ROX (e.g., ~ 97%). While presence ATP, aptamer released from surface, leading recovery fluorescence under 545-nm excitation. Consequently, wide dynamic range 1 μM 1.5 mM high sensitivity limit (LOD) down 0.2 can readily achieved by prepared TC/Apt. further as-prepared TC/Apt probe feasible accurate discrimination different samples including living cells, body fluids mouse serum, urine human serum) tumor models. Conclusions Fluorescence serum), as well model through new nanoprobes, offering powerful tools diseases related abnormal fluctuation concentration.